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We have undertaken a systematic assessment of the behavior of different configurations containing IFN-beta SAR and/or 5'HS4 beta-globin insulator sequences within a gammaretroviral vector optimized for high-level expression, focusing on the long-term achievement of stable, homogeneous transgene expression in the successfully transduced cells.

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We have undertaken a systematic assessment of the behavior of different configurations containing IFN-beta SAR and/or 5'HS4 beta-globin insulator sequences within a gammaretroviral vector optimized for high-level expression, focusing on the long-term achievement of stable, homogeneous transgene expression in the successfully transduced cells.
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CTCF sites are abundant in the genomes of diverse species but their function is enigmatic. We used chromosome conformation capture to determine long-range interactions among CTCF/cohesin sites over 2 Mb on human chromosome 11 encompassing the β-globin locus and flanking olfactory receptor genes. Although CTCF occupies these sites in both erythroid K562 cells and fibroblast 293T cells, the ...
Dec 02, 2015 · Only the capsule structure of a clinical isolate from Thailand, CG8486, that typed as HS4/13/64 has been published . The primer set named HS4 (alpha mix, 370bp) and CG8486 (beta Mix, 652bp) in the first publication were re-named HS4A and HS4B, respectively.
Oct 14, 2004 · Download PDF 20040203158 PDF help. Related US Applications: ... The chicken beta-globin 5′HS4 boundary element blocks enhancer-mediated suppression of silencing ...
May 29, 2013 · HS3 / PRO Beta Releases If this is your first visit, be sure to check out the FAQ. You must register before you can post. Your first post will be checked for appropriate content (SPAM) - please allow a bit of time for that. After that, you'll be able to post at will!
Jan 06, 2014 · However, the HS4-650 in either orientation had higher titer than any other HS4-based LV analyzed (Figure 3B; p<0.01 comparing HS4-650 with HS4-Core and HS4-Ext ). In addition SE-650 expression levels were slightly higher than others HS4s, although it was significant only when compared to SE-ExtFw (p<0.05).
  • Dec 15, 2007 · We next examined the contribution of the flanking regions of the HS4 element, which is a staple of TNS9 8 and other therapeutic globin vectors. 9, –11,13, –15 The T12 vector, which harbors a truncation of the 5′ flanking region of HS4 (Figure 2Ai), showed significantly decreased globin expression both in vivo and in vitro in comparison to ...
  • Connected to the fast USB 2.0, the HS4 doesn’t require an external power supply, therefore the HS4 easily complies to “Plug in and measure”. The universal software that is delivered with the HS4 turns your PC into a storage oscilloscope, a spectrum analyzer, a voltmeter and a transient recorder.
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  • Rewaco Trike HS1 1240 85PS Moto – Acquisto di moto nuove o d'occasione al miglior prezzo. Panoramica di tutti gli annunci nel più grande mercato di moto della Svizzera.
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  • Dec 02, 2015 · Only the capsule structure of a clinical isolate from Thailand, CG8486, that typed as HS4/13/64 has been published . The primer set named HS4 (alpha mix, 370bp) and CG8486 (beta Mix, 652bp) in the first publication were re-named HS4A and HS4B, respectively.
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  • CTCF is a ubiquitous transcription factor that is involved in numerous, seemingly unrelated functions. These functions include, but are not limited to, positive or negative regulation of transcription, enhancer-blocking activities at developmentally regulated gene clusters and at imprinted loci, and X-chromosome inactivation. Here, we review recent data acquired with state-of-the-art ...
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